In the context of the persistent decline in biodiversity and the escalating societal demand for high-quality green spaces, it is essential to foster synergies between biodiversity conservation and the fulfi llment of social requirements within urban parks to optimize their multifunctional advantages. Attaining this objective necessitates a nuanced understanding of public perceptions and preferences concerning urban park biodiversity. In actual park environments, these perceptions and preferences are shaped by interactions among various park attributes. Using Hangzhou as a case study, this research adopted a mixed-methods approach to analyze public perceptions and preferences for urban park biodiversity across multiple attributes. The study comprised two phases. In Phase I, a quantitative analysis was conducted using conjoint analysis to measure public preferences for biodiversity within urban parks. In Phase II, a qualitative study was conducted at Jiangyangfan Park through walk-along interviews, aiming to capture experiential perceptions of biodiversity and to complement and interpret the quantitative fi ndings. The results indicate that the public generally preferred park environments with higher biodiversity, a preference closely associated with perceived benefi ts of biodiversity, including psychological restoration, enhanced environmental quality, and aesthetic value. When making trade-off s among multiple park attributes, the public valued both biodiversity and facilities most, refl ecting a dual demand for naturalness and convenience. There were distinct preference groups among the public, including nature-oriented and recreation-oriented individuals. The former valued ecological experiences and tended to regard artifi cial facilities and intensive vegetation maintenance as disturbances to the natural environment, whereas the latter placed greater emphasis on the comfort and convenience provided by park facilities and were inclined to accept a certain reduction in biodiversity in exchange for improved recreational experiences. These fi ndings provide empirical evidence to inform urban park planning and design strategies that balance biodiversity conservation with public needs.