The flower theme event, an essential form of carrying forward and inheriting flower culture, transmits cultural information by triggering and mobilizing people’s emotions and thoughts and promotes the overall development of the body, mind, and will of groups and individuals. In this paper, a case study was carried out. University students participating in the Daylily Cultural Festival were selected as the research objects. 433 valid questionnaires were collected by questionnaire survey. SPSS 26.0 and AMOS 28.0 were used for descriptive statistical analysis, reliability and validity tests, and structural equation model analysis of the data. The influence path between perception, experience, cultural identity, and supporting behavior in a flower theme event was investigated empirically. The results showed that the periodic theme events had a cumulative effect, and the pre-cognition of the events could promote the cultural cognition of college students. University students’ event experience and cultural experience in the flower theme event positively correlate with their event and cultural cognition. Event experience played an essential role in promoting cultural experience. Excellent event experience and cultural experience complemented each other and promoted cultural identity. The cultural identity of the event brought significant supporting behavior, which proved that the flower theme event played a positive role in the flower culture transmission. Finally, countermeasures and suggestions were put forth from the organization’s perspective, including planning the flower theme event and promoting public value. The study aims to explore how cognition and experience quality affect the cultural identity in flower theme events and provide a theoretical and practical reference for developing flower theme events.