There are many species in the Acanthaceae family with different morphologies, and they have high ornamental value, especially in leaf color, inflorescence, flower color, flowering period, etc. They are widely used in South China. This paper took the species of Acanthaceae collected in Chenshan greenhouse as the research object, based on a few years of cultivation and observation, 34 ornamental species were selected by biological type, ornamental period, ornamental character, and application type. Five application types were showed through planting examples on single planting, natural group planting, vertical, flowerbed, and container planting by biological character and ecological habit. At the same time, the application prospect is envisaged which aims to provide reference for the selection and application of the Acanthaceae plants in different conservatories.