As the precious heritage of Chinese traditional garden culture, the protection and inheritance of Jiangnan garden not only involves material heritage, but also attaches importance to the intangible cultural heritage of garden, especially the protection of intangible cultural heritage construction skills. First of all, this paper reviews the status quo of inheritance of intangible heritage construction skills in Jiangnan gardens, and analyzes the development difficulties faced by intangible heritage construction skills. Secondly, it analyzes the advantages of universities in education, scientific research and resource integration in the protection of intangible cultural heritage construction skills in Jiangnan gardens. Through the three aspects of rescue protection, dynamic inheritance and active dissemination, the research helps transform the inheritance path of Jiangnan garden intangible cultural heritage construction skills, improves students’ skill levels, and makes positive contributions to the sustainable development of Jiangnan garden heritage and the inheritance of intangible cultural heritage skills. Only comprehensive protection and inheritance of intangible heritage construction techniques can ensure the integrity of Jiangnan garden heritage and the permanent inheritance of unique charm.