In the context of rural revitalization, rural research and rural nature education are booming. Rural areas have good natural landscapes and biodiversity resources which become important research spaces for nature education courses. How to protect rural landscape resources while promoting orderly developments of rural nature education is a problem worthy of attention. In this paper, the practice of biodiversity research courses in Jianlou Village, Hunan Province, was demonstrated as an example. And the sustainability of rural landscape resources is used from different perspectives, such as spatial pattern, basic supporting facilities, route design and curriculum planning. At the same time, based on the curriculum practice covering about 500 people, this paper summarizes the relevant experience of activating rural resources, integrating regional routes and integrating into curriculum practice under the guidance of biodiversity research courses, which provides a research and planning way for the protection and utilization of rural landscape resources.