This paper reviews the wilderness conservation cases conducted by the author in urban planning and landscape design projects for more than ten years. It points out that wilderness conservation includes the protection of wilderness land and the protection of wilderness nature of different land use. According to the origin of wilderness, these projects could be classified into four categories: (1) direct transformation of undeveloped wilderness into an urban area; (2) brownfield (industrial land) transformation; (3) abandoned land (disposal of farmland and rural land) rewilding; (4) the exit of artificial management and the entry of natural succession in the process of urban shrinkage. It is proposed to apply field research m, monitoring biodiversity and ecological assessment to construct a landscape ecological assessment decision support system for wilderness biodiversity conservation planning under different planning target systems. Although the awareness and institutional construction of wilderness biodiversity under China’s urbanization process are far from enough, various explorations have been carried out in urban governance. The subjective will of decision-makers at all levels to protect wilderness biodiversity has been growing, which makes the Landscape Ecological Decision and Evaluation Support System (LEDESS) have theoretical and application value in wilderness biodiversity protection planning. From the initial post-urban planning research to the pre-planning pre-research, to the active protection-based urban development planning, to the exploration of active biodiversity conservation in ecological agriculture, in just a few years, the whole society’s understanding of biodiversity has gradually deepened and become more scientific. With more attention and attention after the 15th meeting of the Conference of the Parties to the United Nations Convention on Biological Diversity, more manpower will be devoted to this field, which may usher in the vigorous development of wilderness protection.