The traditional settlement landscape of Zhuang Nationality in northern Guangxi contains the unique cultural order and logic with national characteristics, which is the external spatial expression of the internal law of landscape gene of Southwest Mountain minority in China. Based on the relevant research methods of landscape gene theory, this paper explores the cultural essence of traditional Zhuang villages in mountainous area in northern Guangxi, identifies, extracts and classifies the landscape gene symbols with genetic characteristics, constructs the landscape gene pedigree, and then puts forward the strategies of landscape gene translation and inheritance. The results show that: (1) The landscape gene of traditional Zhuang villages in northern Guangxi is divided into material landscape gene and non-material landscape gene, which can be summarized from five categories: site layout, architectural technology, landscape environment, social customs and cultural art. According to its externalization characteristics, the landscape gene pedigree can be constructed; (2) The landscape gene of mountain minority villages can be translated and inherited from gene purification, gene deconstruction, gene improvement and gene recombination. A systematic summary of the landscape connotation and characteristics of Zhuang villages in northern Guangxi from the perspective of landscape genes is helpful to provide new ideas for the establishment of the protection system and the future development of villages.