As an essential component of Chinese classical gardens, plant landscape accounts for a significant proportion of the garden space. As time goes by, ways to create planting landscape and its characteristics have dramatically changed. The Humble Administrator’s Garden wins by flowers and trees, with a longtime span. Due to the frequent rise and fall of the garden, the plant landscape has significant differences before and after, so it has great research value. Through the analysis and comparison of different literature and images information, this research deduces the relevant information on the plant landscape in each period of Humble Administrator’s Garden, induces its characteristics, clarifies its development context, analyzes the changes of plant landscape before and after and dialyzes the influencing factors of the changes. This article provides research evidence for further researches on the development of plant landscape in Suzhou Scholars’ garden during the Ming and Qing Dynasties.