To identify the occurrence of anthracnose on Ligustrum japonicum in Wuhan and screen out effective fungicides for control of Colletotrichum siamense, the infection was investigated and fungicides susceptibility on C. siamense tested. It demonstrated that latent infection occurred in the anthracnose on L. Japonicum. The early symptoms were not obvious. The damage was aggravating in October, and the incidence reached up to 60~90% at the end of November. The effect of 50% Carbendazim WP and 10% Difenoconazole WG was better than other fungicides, and the value of EC50 of those two fungicides was 0.035 mg/mL and 0.145 mg/mL, respectively. The effect of 25% Triadimefon WP was the worst in those four fungicides; its EC50 value reached 39.396 mg/mL. From the above, Carbendazim and Difenoconazole were the recommended fungicides for preventing anthracnose on L. Japonicum.